1B3L
OLIGO-PEPTIDE BINDING PROTEIN (OPPA) COMPLEXED WITH KGK
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Temperature [K] | 120 |
| Detector technology | IMAGE PLATE |
| Detector | MARRESEARCH |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 69.910, 74.800, 103.770 |
| Unit cell angles | 90.00, 91.10, 90.00 |
Refinement procedure
| Resolution | 15.000 - 2.000 |
| Rwork | 0.178 |
| R-free | 0.24100 |
| Structure solution method | OTHER |
| Starting model (for MR) | 1OLB |
| RMSD bond length | 0.016 |
| RMSD bond angle | 0.035 |
| Data reduction software | DENZO |
| Data scaling software | SCALA |
| Phasing software | CCP4 |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 15.000 | 2.120 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.090 * | 0.188 |
| Number of reflections | 40411 | |
| <I/σ(I)> | 6.6 | 3.4 |
| Completeness [%] | 96.2 | 80.3 |
| Redundancy | 4.3 | 4.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 5.5 | CO-CRYSTALLIZED WITH URANIUM ACETATE, PH 5.5 |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 30 (mg/ml) | |
| 2 | 1 | reservoir | PEG4000 | 5-10 (%(w/v)) | |
| 3 | 1 | reservoir | sodium acetate | 50 (mM) | |
| 4 | 1 | reservoir | uranyl acetate | 1 (mM) |
