1B0T

D15K/K84D MUTANT OF AZOTOBACTER VINELANDII FDI

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU RU200
Temperature [K]	291
Detector technology	AREA DETECTOR
Detector	XENTRONICS
Spacegroup name	P 41 21 2
Unit cell lengths	55.630, 55.630, 95.910
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	8.000 - 2.100
R-factor	0.209
Rwork	0.209
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6fd1
RMSD bond length	0.016
RMSD bond angle	3.700
Data reduction software	X-GEN
Data scaling software	X-GEN
Phasing software	X-PLOR (3.8)
Refinement software	X-PLOR (3.8)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000	2.220
High resolution limit [Å]	2.100	2.100
Number of reflections	8033
<I/σ(I)>	12.4	1.73
Completeness [%]	86.4	43.8
Redundancy	3.13	3.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1		7.8		pH 7.8