Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL7-1 |
| Synchrotron site | SSRL |
| Beamline | BL7-1 |
| Temperature [K] | 277 |
| Detector technology | IMAGE PLATE |
| Detector | MARRESEARCH |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 176.900, 71.900, 73.000 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 20.000 - 2.500 |
| R-factor | 0.193 |
| Rwork | 0.193 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1fgh |
| RMSD bond length | 0.020 |
| RMSD bond angle | 25.000 * |
| Data reduction software | MOSFLM |
| Data scaling software | CCP4 |
| Phasing software | X-PLOR (3.8) |
| Refinement software | X-PLOR (3.8) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 2.630 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Number of reflections | 27625 | |
| <I/σ(I)> | 5.4 | 1.5 |
| Completeness [%] | 84.9 | 83 |
| Redundancy | 2.3 | 1.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion * | 7 | 26 * | pH 7.0 |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 16-18 (mg/ml) | |
| 2 | 1 | drop | Bis-Tris-HCl | 0.25 (M) | |
| 3 | 1 | drop | 0.35 (M) | ||
| 4 | 1 | reservoir | ammonium sulfate | 2.2-2.4 (M) | |
| 5 | 1 | reservoir | bis-Tris-HCl | 0.25 (M) | |
| 6 | 1 | reservoir | 0.35 (M) | ||
| 7 | 1 | drop | ammonium sulfate |
