1B0I

ALPHA-AMYLASE FROM ALTEROMONAS HALOPLANCTIS

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU RU200
Temperature [K]	288
Detector technology	IMAGE PLATE
Detector	MARRESEARCH
Spacegroup name	P 21 21 21
Unit cell lengths	56.800, 80.300, 120.100
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	20.000 - 2.400
R-factor	0.18 *
Rwork	0.180
R-free	0.21700
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	WILDTYPE AHA (1AQH)
RMSD bond length	0.010
RMSD bond angle	1.398 *
Data reduction software	DENZO
Data scaling software	CCP4 ((AGROVATA)
Phasing software	AMoRE
Refinement software	X-PLOR (3.843)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	48.000	2.530
High resolution limit [Å]	2.400	2.400
Rmerge	0.177 *
Total number of observations	128066 *
Number of reflections	21666
<I/σ(I)>	4	1.1
Completeness [%]	98.0	95.8
Redundancy	5.9	5.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	7.5 *		pH 7.0

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	18 (mg/ml)
2	1	drop	ammonium formate	1.7 (M)
3	1	drop	HEPES	0.1 (M)