1B09
HUMAN C-REACTIVE PROTEIN COMPLEXED WITH PHOSPHOCHOLINE
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SRS BEAMLINE PX9.6 |
Synchrotron site | SRS |
Beamline | PX9.6 |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 1995-03-01 |
Detector | MARRESEARCH |
Spacegroup name | P 41 21 2 |
Unit cell lengths | 193.940, 193.940, 134.430 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 20.000 - 2.500 |
Rwork | 0.196 |
R-free | 0.24200 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1sac |
RMSD bond length | 0.010 |
RMSD bond angle | 1.500 |
Data reduction software | MOSFLM |
Data scaling software | CCP4 |
Phasing software | AMoRE |
Refinement software | X-PLOR |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 20.000 | |
High resolution limit [Å] | 2.500 | |
Rmerge | 0.068 | |
Number of reflections | 86545 | |
<I/σ(I)> | 10.1 | |
Completeness [%] | 97.6 | 94.5 * |
Redundancy | 4.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Vapor diffusion, hanging drop * | 7.6 | drop is made up of a 1:1 ratio of protein and reservoir solution * |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | protein | 40 (mg/ml) | |
2 | 1 | reservoir | HEPES | 60 (mM) | |
3 | 1 | reservoir | 140 (mM) | ||
4 | 1 | reservoir | calcium acetate | 10 (mM) | |
5 | 1 | reservoir | MPD | 10 (%(v/v)) |