1B01

TRANSCRIPTIONAL REPRESSOR COPG/DNA COMPLEX

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	EMBL/DESY, HAMBURG BEAMLINE X31
Synchrotron site	EMBL/DESY, HAMBURG
Beamline	X31
Temperature [K]	100
Detector technology	IMAGE PLATE
Detector	MARRESEARCH
Spacegroup name	P 43 2 2
Unit cell lengths	40.300, 40.300, 221.560
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	20.000 - 2.560
R-factor	0.247
Rwork	0.247
R-free	0.31600
Structure solution method	MIRAS
RMSD bond length	0.008
RMSD bond angle	1.450
Data reduction software	MOSFLM
Data scaling software	CCP4 ((SCALA))
Phasing software	CCP4
Refinement software	X-PLOR (3.851)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	19.860 *	1.690
High resolution limit [Å]	2.560 *	2.560 *
Rmerge	0.062 *	0.455 *
Total number of observations	78954 *
Number of reflections	6252 *
<I/σ(I)>	10.3	3
Completeness [%]	96.3 *	86.8 *
Redundancy	5.9 *

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.7		MPD, HEPES, pH 6.70, VAPOR DIFFUSION, HANGING DROP

Crystallization Reagents

ID	crystal ID	solution ID	reagent name	concentration	details
1	1	1	MPD
2	1	1	HEPES
3	1	2	MPD
4	1	2	HEPES

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	reservoir		60-78 (%)
2	1	reservoir		0.1 (M)