1AZX
ANTITHROMBIN/PENTASACCHARIDE COMPLEX
Experimental procedure
| Source type | SYNCHROTRON |
| Source details | SRS BEAMLINE PX9.6 |
| Synchrotron site | SRS |
| Beamline | PX9.6 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 1996-08 |
| Detector | MARRESEARCH |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 70.440, 86.970, 97.220 |
| Unit cell angles | 90.00, 108.88, 90.00 |
Refinement procedure
| Resolution | 30.000 - 2.900 |
| R-factor | 0.203 |
| Rwork | 0.195 |
| R-free | 0.28000 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.005 * |
| RMSD bond angle | 1.000 * |
| Data reduction software | MOSFLM |
| Data scaling software | CCP4 |
| Phasing software | AMoRE |
| Refinement software | TNT (5D) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 30.000 |
| High resolution limit [Å] | 2.900 |
| Rmerge | 0.124 |
| Number of reflections | 60010 |
| <I/σ(I)> | 5.2 |
| Completeness [%] | 94.2 |
| Redundancy | 2.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | unknown * | 7 | 18-20 * | pH 7.0 |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | 1 | anithrombin | 40 (mg/ml) | |
| 2 | 1 | 1 | L-anithrombin | 12.2 (mg/ml) | |
| 3 | 1 | 1 | pentasaccharide | 4 (mg/ml) | |
| 4 | 1 | 1 | PEG4000 | 30 (%(w/v)) | |
| 5 | 1 | 1 | 0.05 (%) | ||
| 6 | 1 | 1 | sodium cacodylate | 0.1 (M) |
