1AZV

FAMILIAL ALS MUTANT G37R CUZNSOD (HUMAN)

Experimental procedure

Source type	ROTATING ANODE
Source details	RIGAKU RUH2R
Temperature [K]	300
Detector technology	IMAGE PLATE
Collection date	1997-01
Detector	RIGAKU
Spacegroup name	P 41
Unit cell lengths	67.200, 67.200, 83.800
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	10.000 - 1.900
R-factor	0.202
Rwork	0.202
R-free	0.24400
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1spd
RMSD bond length	0.011
RMSD bond angle	26.700 *
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	X-PLOR (3.1)
Refinement software	X-PLOR (3.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	100.000	1.970
High resolution limit [Å]	1.900	1.900
Rmerge	0.065 *	0.383 *
Total number of observations	101819 *
Number of reflections	29129
<I/σ(I)>	17	1.8
Completeness [%]	99.1	99.6
Redundancy	3.5	2.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	unknown *	5.5		20 MG/ML PROTEIN IN 10MM ACETATE PH 5.5 MIXED WITH PRECIPITATING SOLUTION OF 100 MM ACETATE PH 4.6, 2.0 M AMMONIUM SULFATE

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	1	protein	10 (mg/ml)
2	1	1	acetate	5 (mM)
3	1	1	sodium acetate	50 (mM)
4	1	1	ammonium sulfate	1.0 (M)
5	1	2	sodium acetate	100 (mM)
6	1	2	ammonium sulfate	2.0 (M)