1AZQ
HYPERTHERMOPHILE CHROMOSOMAL PROTEIN SAC7D BOUND WITH KINKED DNA DUPLEX
Experimental procedure
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU200 |
| Temperature [K] | 298 |
| Detector technology | IMAGE PLATE |
| Collection date | 1997-04-15 |
| Detector | RIGAKU |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 51.765, 77.528, 36.542 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 6.000 - 1.940 |
| R-factor | 0.186 |
| Rwork | 0.186 |
| R-free | 0.23800 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1azp |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.470 |
| Data reduction software | R-AXIS |
| Data scaling software | R-AXIS |
| Phasing software | X-PLOR |
| Refinement software | X-PLOR (3.843) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.000 |
| High resolution limit [Å] | 1.940 | 1.940 |
| Rmerge | 0.057 | 0.280 |
| Number of reflections | 10085 | |
| <I/σ(I)> | 14.2 | 1.8 |
| Completeness [%] | 87.9 | 65.8 |
| Redundancy | 5.2 | 2.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | pH 6.5, VAPOR DIFFUSION, HANGING DROP |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | PEG 400 | ||
| 2 | 1 | 1 | TRIS BUFFER | ||
| 3 | 1 | 2 | PEG 400 |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 1.3 (mM) | |
| 2 | 1 | drop | DNA duplex | 1.3 (mM) | |
| 3 | 1 | drop | Tris-HCl | 2 (mM) | |
| 4 | 1 | drop | PEG400 | 2.6 (%) | |
| 5 | 1 | reservoir | PEG400 | 15 (%) |
