1AZN
CRYSTAL STRUCTURE OF THE AZURIN MUTANT PHE114ALA FROM PSEUDOMONAS AERUGINOSA AT 2.6 ANGSTROMS RESOLUTION
Experimental procedure
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 51.000, 83.600, 66.400 |
| Unit cell angles | 90.00, 110.50, 90.00 |
Refinement procedure
| Resolution | 8.000 - 2.600 |
| R-factor | 0.185 |
| Rwork | 0.185 |
| RMSD bond length | 0.020 |
| RMSD bond angle | 1.740 |
| Phasing software | X-PLOR |
| Refinement software | X-PLOR |
Data quality characteristics
| Overall | |
| High resolution limit [Å] | 2.600 * |
| Number of reflections | 14831 * |
| Completeness [%] | 91.0 * |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 6 * | 297-298 * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | reservoir | PEG4000 | 25 (%) | |
| 2 | 1 | reservoir | calcium chloride | 0.2 (M) | |
| 3 | 1 | reservoir | acetate | 0.22 (M) | |
| 4 | 1 | drop | protein | 7.5-10 (mg/ml) |
