1AZ5
UNLIGANDED SIV PROTEASE STRUCTURE IN AN "OPEN" CONFORMATION
Experimental procedure
| Temperature [K] | 298 |
| Detector technology | IMAGE PLATE |
| Collection date | 1994-10 |
| Detector | RIGAKU |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 45.400, 45.400, 87.900 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 7.000 - 2.000 |
| R-factor | 0.204 |
| Rwork | 0.204 |
| R-free | 0.26600 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1SAM |
| RMSD bond length | 0.011 |
| RMSD bond angle | 27.900 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | X-PLOR |
| Refinement software | X-PLOR |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.060 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.086 | |
| Number of reflections | 7300 | |
| Completeness [%] | 97.0 | 94 |
| Redundancy | 5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 6.5 | 100 MM SODIUM CACODYLATE, PH 6.5, 0.3 M NACL |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | reservoir | 4-6 (%sat) | ||
| 2 | 1 | reservoir | sodium cacodylate | 100 (mM) |
