1AZ4

ECORV ENDONUCLEASE, UNLIGANDED, FORM B, T93A MUTANT

Experimental procedure

Temperature [K]	293
Collection date	1996-09
Spacegroup name	P 21 21 21
Unit cell lengths	74.000, 121.700, 59.300
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	6.000 - 2.400
R-factor	0.225
Rwork	0.225
R-free	0.33700
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.011
RMSD bond angle	24.000 *
Phasing software	X-PLOR
Refinement software	X-PLOR

Data quality characteristics

	Overall
High resolution limit [Å]	2.400
Rmerge	0.085 *

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, sitting drop *	7.5		pH 7.5

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	8.8 (mg/ml)
2	1	reservoir	PEG4000	8 (%)