1AZ2
CITRATE BOUND, C298A/W219Y MUTANT HUMAN ALDOSE REDUCTASE
Experimental procedure
| Source type | ROTATING ANODE |
| Source details | ELLIOTT GX-6 |
| Temperature [K] | 277 |
| Detector technology | AREA DETECTOR |
| Collection date | 1994-08 |
| Detector | SIEMENS |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 50.034, 67.167, 92.177 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 10.000 - 2.900 |
| R-factor | 0.196 |
| Rwork | 0.196 |
| Structure solution method | DIFFERENCE FOURIER |
| Starting model (for MR) | 2acs |
| RMSD bond length | 0.013 * |
| RMSD bond angle | 1.400 * |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | X-PLOR |
| Refinement software | X-PLOR |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.000 | 3.000 |
| High resolution limit [Å] | 2.900 | 2.900 |
| Rmerge | 0.128 * | 0.225 * |
| Total number of observations | 31641 * | |
| Number of reflections | 7308 | 1288 * |
| Redundancy | 4.3 | 3.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 5 * | 4 * | pH 6.5 |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | reservoir | PEG6000 | 15-20 (%(w/w)) | |
| 2 | 1 | drop | protein | 14 (mg/ml) | |
| 3 | 1 | drop | PEG6000 | 15-20 (%(w/w)) | |
| 4 | 1 | drop | sodium citrate | 25 (mM) | |
| 5 | 1 | drop | beta-mercaptoethanol | 7 (mM) | |
| 6 | 1 | drop | NADP+ | 1 (mM) | |
| 7 | 1 | drop | Alrestatin | 1 (mM) |
