1AZ0
ECORV ENDONUCLEASE/DNA COMPLEX
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU200 |
| Temperature [K] | 293 |
| Detector technology | IMAGE PLATE |
| Collection date | 1995-08-15 |
| Detector | RIGAKU RAXIS IIC |
| Spacegroup name | P 1 |
| Unit cell lengths | 49.000, 50.200, 64.100 |
| Unit cell angles | 109.00, 108.00, 96.00 |
Refinement procedure
| Resolution | 6.000 - 2.000 |
| R-factor | 0.184 |
| Rwork | 0.184 |
| R-free | 0.24600 |
| RMSD bond length | 0.013 |
| RMSD bond angle | 23.500 * |
| Data reduction software | DENZO |
| Data scaling software | R-AXIS |
| Phasing software | X-PLOR |
| Refinement software | X-PLOR |
Data quality characteristics
| Overall | |
| High resolution limit [Å] | 2.000 |
| Rmerge | 0.065 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6 | 293 | pH 6.00, VAPOR DIFFUSION, SITTING DROP, temperature 293.00K |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | PEG 4000 | ||
| 2 | 1 | 1 | NACL | ||
| 3 | 1 | 1 | K3PO4 | ||
| 4 | 1 | 1 | EDTA | ||
| 5 | 1 | 1 | CACL2 | ||
| 6 | 1 | 2 | NACL | ||
| 7 | 1 | 2 | PEG 4000 |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | 40 (mM) | ||
| 2 | 1 | reservoir | PEG4000 | 1-2 (%) |
