1AYR
ARRESTIN FROM BOVINE ROD OUTER SEGMENTS
Experimental procedure
| Source type | SYNCHROTRON |
| Source details | SRS BEAMLINE PX9.5 |
| Synchrotron site | SRS |
| Beamline | PX9.5 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 1996-04 |
| Detector | MARRESEARCH |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 169.170, 185.530, 90.930 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 130.000 - 3.300 |
| R-factor | 0.254 |
| Rwork | 0.254 |
| R-free | 0.31600 |
| Structure solution method | MIRAS |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.200 |
| Data reduction software | DENZO |
| Data scaling software | CCP4 ((SCALA)) |
| Phasing software | X-PLOR (3.851) |
| Refinement software | X-PLOR (3.851) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 26.976 | 3.390 |
| High resolution limit [Å] | 3.300 | 3.300 |
| Rmerge | 0.075 | 0.172 |
| Number of reflections | 478820 | |
| <I/σ(I)> | 8.6 | 4.3 |
| Completeness [%] | 95.1 | 79.7 |
| Redundancy | 4.9 | 2.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 7.2 | pH 7.2 |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 36 (mg/ml) | |
| 10 | 1 | reservoir | ethylene glycol | 0.02 (%) | |
| 11 | 1 | reservoir | PEG6000 | 5.7 (%) | |
| 12 | 1 | reservoir | PEG200 | 13 (%) | |
| 13 | 1 | reservoir | PEG1000 | 4 (%) | |
| 2 | 1 | drop | PIPES | 18 (mM) | |
| 3 | 1 | drop | 0.5 (M) | ||
| 4 | 1 | drop | ethylene glycol | 30 (%) | |
| 5 | 1 | drop | PEG6000 | 8 (%) | |
| 6 | 1 | drop | PEG200 | 18 (%) | |
| 7 | 1 | drop | PEG1000 | 6 (%) | |
| 8 | 1 | reservoir | PIPES | 40 (mM) | |
| 9 | 1 | reservoir | 0.6 (M) |
