1AYE
HUMAN PROCARBOXYPEPTIDASE A2
Experimental procedure
| Source type | ROTATING ANODE |
| Source details | RIGAKU RUH2R |
| Temperature [K] | 288 |
| Detector technology | IMAGE PLATE |
| Collection date | 1997-04 |
| Detector | MARRESEARCH |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 42.200, 87.070, 59.020 |
| Unit cell angles | 90.00, 99.35, 90.00 |
Refinement procedure
| Resolution | 8.000 - 1.800 |
| R-factor | 0.178 |
| Rwork | 0.178 |
| R-free | 0.22200 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1pca |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.736 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | X-PLOR (3.843) |
| Refinement software | X-PLOR (3.843) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 1.860 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Rmerge | 0.047 | 0.178 |
| Number of reflections | 38772 | |
| <I/σ(I)> | 32.6 | 9.1 |
| Completeness [%] | 94.0 | 87.9 |
| Redundancy | 3.4 | 3.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | unknown * | 8 | 4 * | pH 8.0 |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | 1 | PEG | 20 (%) | |
| 2 | 1 | 1 | nickel chloride | 0.01 (M) | |
| 3 | 1 | 1 | Tris-HCl | 0.1 (M) |
