1AYD

CRYSTAL STRUCTURES OF PEPTIDE COMPLEXES OF THE AMINO-TERMINAL SH2 DOMAIN OF THE SYP TYROSINE PHOSPHATASE

Experimental procedure

Spacegroup name	P 43 21 2
Unit cell lengths	63.200, 63.200, 76.100
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	6.000 - 2.200
R-factor	0.2
Rwork	0.200
RMSD bond length	0.012
RMSD bond angle	3.000
Phasing software	X-PLOR
Refinement software	X-PLOR

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000 *
High resolution limit [Å]	2.200 *	2.200 *
Rmerge	0.053 *
Total number of observations	63420 *
Number of reflections	8075 *
Completeness [%]	97.0 *	92.1 *

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	6 *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	reservoir	ammonium sulfate	50 (%sat)
2	1	reservoir	MES	100 (mM)