1AY7
RIBONUCLEASE SA COMPLEX WITH BARSTAR
Experimental procedure
| Source type | SYNCHROTRON |
| Source details | EMBL/DESY, HAMBURG BEAMLINE X31 |
| Synchrotron site | EMBL/DESY, HAMBURG |
| Beamline | X31 |
| Temperature [K] | 293 |
| Detector technology | IMAGE PLATE |
| Collection date | 1996-11 |
| Detector | MARRESEARCH |
| Spacegroup name | P 65 |
| Unit cell lengths | 56.950, 56.950, 135.800 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 25.000 - 1.700 |
| R-factor | 0.162 |
| Rwork | 0.160 |
| R-free | 0.19000 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1brs |
| RMSD bond length | 0.025 |
| RMSD bond angle | 0.040 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 25.000 | 1.720 |
| High resolution limit [Å] | 1.700 | 1.700 |
| Rmerge | 0.046 | 0.308 |
| Total number of observations | 118438 * | |
| Number of reflections | 22844 * | |
| <I/σ(I)> | 27.3 | 3.7 |
| Completeness [%] | 100.0 | 99.8 |
| Redundancy | 4.7 | 4.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion * | 7.2 * | pH 7.0 |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 20 (mg/ml) | |
| 2 | 1 | drop | cacodylate | 0.1 (M) | |
| 3 | 1 | drop | ammonium sulfate | 15 (%) | |
| 4 | 1 | reservoir | ammonium sulfate | 30 (%) |
