1AX8
Human obesity protein, leptin
Experimental procedure
| Source type | ROTATING ANODE |
| Source details | RIGAKU RUH2R |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 1996-09 |
| Detector | RIGAKU |
| Spacegroup name | P 63 |
| Unit cell lengths | 88.140, 88.140, 48.050 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 8.000 - 2.400 |
| R-factor | 0.185 |
| Rwork | 0.185 |
| R-free | 0.28300 |
| Structure solution method | MIR |
| RMSD bond length | 0.009 |
| RMSD bond angle | 20.380 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | MLPHARE |
| Refinement software | X-PLOR (3.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 15.000 | 2.440 |
| High resolution limit [Å] | 2.400 | 2.400 |
| Rmerge | 0.052 | 0.221 |
| Total number of observations | 45300 * | |
| Number of reflections | 8404 | |
| <I/σ(I)> | 14.5 | 8.5 |
| Completeness [%] | 99.2 | 99.5 |
| Redundancy | 5.4 | 4.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | unknown * | 7.5 | PROTEIN WAS CRYSTALLIZED FROM 1.8M NA FORMATE, 100MM TRIS, PH 7.5 |
