1AX4
TRYPTOPHANASE FROM PROTEUS VULGARIS
Experimental procedure
| Source type | SYNCHROTRON |
| Source details | EMBL/DESY, HAMBURG BEAMLINE BW7B |
| Synchrotron site | EMBL/DESY, HAMBURG |
| Beamline | BW7B |
| Temperature [K] | 293 |
| Detector technology | IMAGE PLATE |
| Collection date | 1994-01 |
| Detector | MARRESEARCH |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 114.990, 118.230, 153.680 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 18.000 - 2.100 |
| R-factor | 0.187 * |
| Rwork | 0.187 |
| R-free | 0.22749 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | TYROSINE PHENOL LYASE PDB ENTRY 1TPL |
| RMSD bond length | 0.012 * |
| RMSD bond angle | 0.030 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 18.000 | 2.170 |
| High resolution limit [Å] | 2.100 | 2.100 |
| Rmerge | 0.098 | 0.273 |
| Total number of observations | 397510 * | |
| Number of reflections | 117863 * | |
| <I/σ(I)> | 11 | 3.2 |
| Completeness [%] | 97.6 | 97.3 |
| Redundancy | 3.33 | 2.49 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 7.8 | 27 % PEG 4000, 0.1 M POTASSIUM PHOSPHATE BUFFER PH 7.8, 0.25 MM PYRIDOXAL 5'-PHOSPHATE, 5MM DTT, 0.1 M CSCL. |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | reservoir | PLP | 0.25 (mM) | |
| 2 | 1 | reservoir | dithiothreitol | 5 (mM) | |
| 3 | 1 | reservoir | 0.1 (M) | ||
| 4 | 1 | reservoir | potassium phosphate | 0.1 (M) | |
| 5 | 1 | reservoir | PEG4000 | 27 (%) |
