1AWP
RAT OUTER MITOCHONDRIAL MEMBRANE CYTOCHROME B5
Experimental procedure
| Temperature [K] | 293 |
| Detector technology | AREA DETECTOR |
| Collection date | 1997-06 |
| Detector | SIEMENS |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 45.640, 71.190, 73.780 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 8.000 - 2.000 |
| R-factor | 0.1802 |
| Rwork | 0.180 |
| R-free | 0.22590 * |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1b5m |
| RMSD bond length | 0.007 |
| RMSD bond angle | 24.200 * |
| Data reduction software | SAINT |
| Data scaling software | SAINT |
| Phasing software | X-PLOR (3.851) |
| Refinement software | X-PLOR (3.851) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 10.000 | 2.070 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.075 * | 0.274 * |
| Number of reflections | 16374 | |
| <I/σ(I)> | 32.42 | 3.41 |
| Completeness [%] | 97.7 | 84.7 * |
| Redundancy | 3.2 | 2.18 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion * | 7.8 * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | TRIS | 20 (mM) | |
| 2 | 1 | drop | sodium azide | 0.1 (%) | |
| 3 | 1 | drop | protein | 20 (mg/ml) | |
| 4 | 1 | reservoir | PEG8000 MW | 30 (%) | |
| 5 | 1 | reservoir | magnesium acetate | 0.20 (M) | |
| 6 | 1 | reservoir | PIPES | 0.10 (M) |
