1AWP

RAT OUTER MITOCHONDRIAL MEMBRANE CYTOCHROME B5

Experimental procedure

Temperature [K]	293
Detector technology	AREA DETECTOR
Collection date	1997-06
Detector	SIEMENS
Spacegroup name	P 21 21 21
Unit cell lengths	45.640, 71.190, 73.780
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	8.000 - 2.000
R-factor	0.1802
Rwork	0.180
R-free	0.22590 *
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1b5m
RMSD bond length	0.007
RMSD bond angle	24.200 *
Data reduction software	SAINT
Data scaling software	SAINT
Phasing software	X-PLOR (3.851)
Refinement software	X-PLOR (3.851)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	10.000	2.070
High resolution limit [Å]	2.000	2.000
Rmerge	0.075 *	0.274 *
Number of reflections	16374
<I/σ(I)>	32.42	3.41
Completeness [%]	97.7	84.7 *
Redundancy	3.2	2.18

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion *	7.8 *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	TRIS	20 (mM)
2	1	drop	sodium azide	0.1 (%)
3	1	drop	protein	20 (mg/ml)
4	1	reservoir	PEG8000 MW	30 (%)
5	1	reservoir	magnesium acetate	0.20 (M)
6	1	reservoir	PIPES	0.10 (M)