1AWC
MOUSE GABP ALPHA/BETA DOMAIN BOUND TO DNA
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X4A |
| Synchrotron site | NSLS |
| Beamline | X4A |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 1996-05-15 |
| Detector | FUJI |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 201.600, 34.550, 59.260 |
| Unit cell angles | 90.00, 99.83, 90.00 |
Refinement procedure
| Resolution | 6.000 - 2.150 |
| R-factor | 0.211 * |
| Rwork | 0.211 |
| R-free | 0.28200 |
| Structure solution method | MIR |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.171 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | MLPHARE |
| Refinement software | X-PLOR (3.8) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.230 |
| High resolution limit [Å] | 2.150 | 2.150 |
| Rmerge | 0.072 * | 0.220 * |
| Number of reflections | 18368 | |
| <I/σ(I)> | 21 | 5.3 |
| Completeness [%] | 88.0 | 88 |
| Redundancy | 8.7 | 7.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 9 | 20 * | 100 MM BIS-TRIS PROPANE PH 9, 12 % PEG 1000, 5 MM COBALTIC HEXAMINE CHLORIDE, 1 MM DTT, 20 MM TRIS, 1 MM EDTA, 0.001 % SODIUM AZIDE, pH 9.0 |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | BIS-TRIS PROPANE | ||
| 2 | 1 | 1 | PEG 1000 | ||
| 3 | 1 | 1 | [CO(NH3)6]Cl3 | ||
| 4 | 1 | 1 | DTT | ||
| 5 | 1 | 1 | TRIS | ||
| 6 | 1 | 1 | EDTA | ||
| 7 | 1 | 1 | SODIUM AZIDE | ||
| 8 | 1 | 2 | PEG 1000 |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 4 (mg/ml) | |
| 10 | 1 | reservoir | cobaltic hexamine chloride | 5 (mM) | |
| 11 | 1 | reservoir | PEG1000 | 9 (%) | |
| 2 | 1 | drop | Tris | 10 (mM) | |
| 3 | 1 | drop | EDTA | 0.5 (mM) | |
| 4 | 1 | drop | dithiothreitol | 0.5 (mM) | |
| 5 | 1 | drop | sodium azide | 0.0005 (%) | |
| 6 | 1 | drop | Bis-Tris propane | 50 (mM) | |
| 7 | 1 | drop | cobaltic hexamine chloride | 2.5 (mM) | |
| 8 | 1 | drop | PEG1000 | 4.5 (%) | |
| 9 | 1 | reservoir | Bis-Tris propane | 100 (mM) |
