1AVO
PROTEASOME ACTIVATOR REG(ALPHA)
Experimental procedure
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL9-1 |
| Synchrotron site | SSRL |
| Beamline | BL9-1 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 1997-02 |
| Detector | MARRESEARCH |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 162.600, 134.300, 116.200 |
| Unit cell angles | 90.00, 90.60, 90.00 |
Refinement procedure
| Resolution | 10.000 - 2.800 |
| R-factor | 0.249 |
| Rwork | 0.249 |
| R-free | 0.28900 |
| Structure solution method | MIR/AVERAGING |
| RMSD bond length | 0.025 |
| RMSD bond angle | 2.100 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | X-PLOR (3.8) |
| Refinement software | X-PLOR (3.8) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.850 |
| High resolution limit [Å] | 2.800 | 2.800 |
| Rmerge | 0.087 | 0.241 |
| Number of reflections | 32245 | |
| <I/σ(I)> | 17 | 9 |
| Completeness [%] | 98.4 | 95 |
| Redundancy | 8.9 | 2.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, sitting drop * | 7.2 * | 4 * | pH 7.1 |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 5 (mg/ml) | |
| 10 | 1 | reservoir | Na-MOPS | 100 (mM) | |
| 11 | 1 | reservoir | 2 (M) | ||
| 12 | 1 | reservoir | 0.9 (mM) | ||
| 2 | 1 | drop | Mops | 5 (mM) | |
| 3 | 1 | drop | EDTA | 0.1 (mM) | |
| 4 | 1 | drop | dithiothreitol | 0.5 (mM) | |
| 5 | 1 | drop | PEG6000 | 6 (%) | |
| 6 | 1 | drop | Na-MOPS | 50 (mM) | |
| 7 | 1 | drop | 1 (M) | ||
| 8 | 1 | drop | 0.45 (mM) | ||
| 9 | 1 | reservoir | PEG6000 | 12 (%) |
