1AVL

CRYSTAL STRUCTURES OF THE COPPER-CONTAINING AMINE OXIDASE FROM ARTHROBACTER GLOBIFORMIS IN THE HOLO-AND APO-FORMS: IMPLICATIONS FOR THE BIOGENESIS OF TOPA QUINONE

Experimental procedure

Source type	ROTATING ANODE
Source details	RIGAKU RUH2R
Temperature [K]	293
Detector technology	IMAGE PLATE
Collection date	1995-12
Detector	RIGAKU RAXIS II
Spacegroup name	C 1 2 1
Unit cell lengths	158.200, 64.100, 92.900
Unit cell angles	90.00, 112.70, 90.00

Refinement procedure

Resolution	50.000 - 2.800
R-factor	0.15
Rwork	0.150
R-free	0.20200
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	HOLO-ACTIVE FORM
RMSD bond length	0.011
RMSD bond angle	1.700 *
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	X-PLOR (3.8)
Refinement software	X-PLOR (3.8)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.900
High resolution limit [Å]	2.800	2.800
Rmerge	0.082 *
Total number of observations	33361 *
Number of reflections	19006
Completeness [%]	88.7	88.8
Redundancy	1.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	8.7		pH 8.7

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	reservoir	ammonium salfate	1.0 (M)
2	1	reservoir	PEG8000	10 (%(w/v))
3	1	reservoir	Tris-HCl	0.1 (M)	pH8.7