1AVK

CRYSTAL STRUCTURES OF THE COPPER-CONTAINING AMINE OXIDASE FROM ARTHROBACTER GLOBIFORMIS IN THE HOLO-AND APO-FORMS: IMPLICATIONS FOR THE BIOGENESIS OF TOPA QUINONE

Experimental procedure

Source type	ROTATING ANODE
Source details	RIGAKU RUH2R
Temperature [K]	293
Detector technology	IMAGE PLATE
Collection date	1995-12
Detector	RIGAKU
Spacegroup name	C 1 2 1
Unit cell lengths	158.720, 64.600, 93.290
Unit cell angles	90.00, 112.33, 90.00

Refinement procedure

Resolution	50.000 - 2.200
R-factor	0.194
Rwork	0.194
R-free	0.23700
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	HOLO-AGAO
RMSD bond length	0.009
RMSD bond angle	1.700 *
Data reduction software	PROCESS
Data scaling software	PROCESS
Phasing software	AMoRE
Refinement software	X-PLOR (3.8)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.300
High resolution limit [Å]	2.200	2.200
Rmerge	0.061	0.208
Total number of observations	58200 *
Number of reflections	39585
Completeness [%]	87.3	87.7
Redundancy	1.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	6.8		pH 6.8

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	reservoir	potassium sodium tartrate	0.2 (M)
2	1	reservoir	HEPES	25 (mM)	pH6.8