1AVA
AMY2/BASI PROTEIN-PROTEIN COMPLEX FROM BARLEY SEED
Experimental procedure
| Source type | ROTATING ANODE |
| Source details | RIGAKU RUH2R |
| Temperature [K] | 293 |
| Detector technology | IMAGE PLATE |
| Collection date | 1994-03 |
| Detector | MARRESEARCH |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 74.500, 96.180, 170.150 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 8.000 - 1.900 |
| R-factor | 0.208 |
| Rwork | 0.208 |
| R-free | 0.26900 |
| Structure solution method | PHASE COMBINATION |
| Starting model (for MR) | AMY2 |
| RMSD bond length | 0.007 |
| RMSD bond angle | 25.300 * |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | X-PLOR (3.1) |
| Refinement software | X-PLOR (3.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 15.000 | 2.100 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Number of reflections | 95528 * | |
| Completeness [%] | 92.0 | 82.5 |
| Redundancy | 8.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion * | 6.7 | 20 * | pH 6.7 |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 9.4 (mg/ml) | |
| 2 | 1 | reservoir | PEG6000 | 3-8 (%) | |
| 3 | 1 | reservoir | MES | 10 (mM) | |
| 4 | 1 | reservoir | 5 (mM) |
