1AV6
VACCINIA METHYLTRANSFERASE VP39 COMPLEXED WITH M7G CAPPED RNA HEXAMER AND S-ADENOSYLHOMOCYSTEINE
Experimental procedure
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU200 |
| Temperature [K] | 103 |
| Detector technology | AREA DETECTOR |
| Collection date | 1997-08-15 |
| Detector | SIEMENS |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 61.800, 64.600, 99.500 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 8.000 - 2.800 |
| R-factor | 0.215 |
| Rwork | 0.215 |
| R-free | 0.28500 |
| Structure solution method | MOLECULAR |
| Starting model (for MR) | 1vpt |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.700 |
| Data reduction software | SMART |
| Data scaling software | SAINT |
| Phasing software | AMoRE |
| Refinement software | X-PLOR (3.851) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 2.800 |
| High resolution limit [Å] | 2.700 | 2.700 |
| Rmerge | 0.095 | 0.210 |
| Total number of observations | 22144 * | |
| Number of reflections | 9755 | |
| <I/σ(I)> | 20 | 6 |
| Completeness [%] | 92.0 | |
| Redundancy | 3.0 * | 2.0 * |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 7 * | used to seeding * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | reservoir | PEG8000 | 15-20 (%) | |
| 2 | 1 | reservoir | cacodylate | 0.1 (M) | pH7.0 |
| 3 | 1 | reservoir | ammonium sulfate | 0.125 (M) | |
| 4 | 1 | drop | delta C26 | 0.26 (mM) | |
| 5 | 1 | drop | RNA | 0.9 (mM) | |
| 6 | 1 | drop | AdoHcy | 1 (mM) |
