1AUX

STRUCTURE OF THE C DOMAIN OF SYNAPSIN IA FROM BOVINE BRAIN WITH CALCIUM ATP-GAMMA-S BOUND

Experimental procedure

Source type	ROTATING ANODE
Source details	RIGAKU RUH3R
Temperature [K]	140
Detector technology	IMAGE PLATE
Collection date	1996-07
Detector	RIGAKU RAXIS IIC
Spacegroup name	P 32 2 1
Unit cell lengths	76.160, 76.160, 182.330
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	20.000 - 2.300
R-factor	0.206
Rwork	0.206
R-free	0.26900
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1auv
RMSD bond length	0.011
RMSD bond angle	24.600 *
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	X-PLOR (3.851)
Refinement software	X-PLOR (3.851)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000	2.370
High resolution limit [Å]	2.200	2.300
Rmerge	0.053	0.210
Total number of observations	88952 *
Number of reflections	28177
<I/σ(I)>	15.5	3.2
Completeness [%]	88.0	71.7
Redundancy	3.2	1.44

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	7.2 *		Wang, C.R., (1997) Protein Sci., 6, 2264. *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	PEG4000	6 (%)
2	1	drop	Tris-HCl	100 (mM)
3	1	reservoir		0.3 (M)