1AUV

STRUCTURE OF THE C DOMAIN OF SYNAPSIN IA FROM BOVINE BRAIN

Experimental procedure

Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X4A
Synchrotron site	NSLS
Beamline	X4A
Temperature [K]	130
Detector technology	IMAGE PLATE
Collection date	1995-10
Detector	FUJI
Spacegroup name	P 32 2 1
Unit cell lengths	76.400, 76.400, 180.940
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	30.000 - 2.150
R-factor	0.199
Rwork	0.199
R-free	0.25500
Structure solution method	MIR
RMSD bond length	0.012
RMSD bond angle	24.600 *
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	MLPHARE
Refinement software	X-PLOR (3.851)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	2.220
High resolution limit [Å]	2.150	2.150
Rmerge	0.048	0.113
Total number of observations	105390 *
Number of reflections	55873
<I/σ(I)>	14.6	4.2
Completeness [%]	74.3	48
Redundancy	1.9	1.58

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	7.2 *	299	Wang, C.R., (1997) Protein Sci., 6, 2264. *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	PEG4000	6 (%)
2	1	drop	Tris-HCl	100 (mM)
3	1	reservoir		0.3 (M)