1AUT

Human activated protein C

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU
Temperature [K]	285
Detector technology	IMAGE PLATE
Collection date	1995-02-05
Detector	MARRESEARCH
Spacegroup name	P 21 21 21
Unit cell lengths	57.060, 89.600, 101.230
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	8.000 - 2.800
R-factor	0.184 *
Rwork	0.184
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	FACTOR XA POLY-ALA
RMSD bond length	0.012
RMSD bond angle	2.072
Data reduction software	DENZO
Phasing software	MAIN (V. 95)
Refinement software	PROLSQ

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	8.000	2.900
High resolution limit [Å]	2.800	2.800
Rmerge	0.126
Number of reflections	12294
Completeness [%]	92.4	85.5
Redundancy	2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	4.7		0.1 M CITRATE, 20% ISOPROPANOL 2% PEG 20000 PH 4.7

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	reservoir	PEG10000	2 (%)
2	1	reservoir	isopropanol	20 (%)
3	1	reservoir	citrate	0.1 (M)