1AUS
ACTIVATED UNLIGANDED SPINACH RUBISCO
Experimental procedure
| Source type | SYNCHROTRON |
| Source details | SRS BEAMLINE PX9.6 |
| Synchrotron site | SRS |
| Beamline | PX9.6 |
| Detector technology | IMAGE PLATE |
| Collection date | 1994-03-28 |
| Detector | MARRESEARCH |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 157.400, 158.700, 203.000 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 7.000 - 2.200 |
| R-factor | 0.207 * |
| Rwork | 0.217 |
| R-free | 0.22400 * |
| RMSD bond length | 0.007 * |
| RMSD bond angle | 1.667 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | X-PLOR |
| Refinement software | X-PLOR |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 20.000 |
| High resolution limit [Å] | 2.200 * |
| Rmerge | 0.103 * |
| Total number of observations | 799161 * |
| Number of reflections | 107965 * |
| Completeness [%] | 85.0 * |
| Redundancy | 6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 4 * | Taylor, T.C., (1996) Nat. Struct. Biol., 3, 95. * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 50-60 (mg/ml) | |
| 2 | 1 | drop | 3-PGA | 100 (mM) | |
| 3 | 1 | reservoir | PEG4000 | 14-16 (%) | |
| 4 | 1 | reservoir | 0.2 (M) | ||
| 5 | 1 | reservoir | HEPES | 25 (mM) | |
| 6 | 1 | reservoir | 10 (mM) | ||
| 7 | 1 | reservoir | 50 (mM) | ||
| 8 | 1 | reservoir | 0.02 (%) |
