1AUS

ACTIVATED UNLIGANDED SPINACH RUBISCO

Experimental procedure

Source type	SYNCHROTRON
Source details	SRS BEAMLINE PX9.6
Synchrotron site	SRS
Beamline	PX9.6
Detector technology	IMAGE PLATE
Collection date	1994-03-28
Detector	MARRESEARCH
Spacegroup name	C 2 2 21
Unit cell lengths	157.400, 158.700, 203.000
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	7.000 - 2.200
R-factor	0.207 *
Rwork	0.217
R-free	0.22400 *
RMSD bond length	0.007 *
RMSD bond angle	1.667 *
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	X-PLOR
Refinement software	X-PLOR

Data quality characteristics

	Overall
Low resolution limit [Å]	20.000
High resolution limit [Å]	2.200 *
Rmerge	0.103 *
Total number of observations	799161 *
Number of reflections	107965 *
Completeness [%]	85.0 *
Redundancy	6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *		4 *	Taylor, T.C., (1996) Nat. Struct. Biol., 3, 95. *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	50-60 (mg/ml)
2	1	drop	3-PGA	100 (mM)
3	1	reservoir	PEG4000	14-16 (%)
4	1	reservoir		0.2 (M)
5	1	reservoir	HEPES	25 (mM)
6	1	reservoir		10 (mM)
7	1	reservoir		50 (mM)
8	1	reservoir		0.02 (%)