1AUQ

A1 DOMAIN OF VON WILLEBRAND FACTOR

Experimental procedure

Source type	ROTATING ANODE
Source details	RIGAKU
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	1997-06
Detector	RIGAKU
Spacegroup name	P 61
Unit cell lengths	86.395, 86.395, 68.125
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	15.000 - 2.300
R-factor	0.186
Rwork	0.186
R-free	0.24800
Structure solution method	MIR, MR
Starting model (for MR)	ALPHA2 I DOMAIN
RMSD bond length	0.011
RMSD bond angle	25.700 *
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	X-PLOR (3.843)
Refinement software	X-PLOR (3.843)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	10.000 *	2.380
High resolution limit [Å]	2.300	2.300
Rmerge	0.083 *
Number of reflections	11849
<I/σ(I)>	13.2	2.4
Completeness [%]	91.1	61.1
Redundancy	4.4	1.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	8.5		0.1M TRIS PH 8.5, 5MM CDCL2, 5% PEG 8K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	reservoir	Tris	0.1 (M)
2	1	reservoir	PEG8000	8 (%)
3	1	reservoir		5 (mM)