1AUA
PHOSPHATIDYLINOSITOL TRANSFER PROTEIN SEC14P FROM SACCHAROMYCES CEREVISIAE
Experimental procedure
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL7-1 |
| Synchrotron site | SSRL |
| Beamline | BL7-1 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 1997-02 |
| Detector | MARRESEARCH |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 88.971, 88.971, 111.339 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 30.000 - 2.500 |
| R-factor | 0.211 |
| Rwork | 0.211 |
| R-free | 0.27300 |
| Structure solution method | MIR |
| RMSD bond length | 0.008 |
| RMSD bond angle | 24.080 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | MLPHARE |
| Refinement software | X-PLOR (3.85) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.670 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Rmerge | 0.051 * | 0.265 * |
| Number of reflections | 16736 | |
| <I/σ(I)> | 20 | 8 |
| Completeness [%] | 90.8 | 80 |
| Redundancy | 3 | 2.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 7.2 | 3% PEG 3350, 1% N-OCTYL B-D-GLUCOPYRANOSIDE, 10 MM HEPES, 150MM KCL, PH 7.2 |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 20 (mg/ml) | |
| 2 | 1 | drop | HEPES | 10 (mM) | |
| 3 | 1 | drop | 150 (mM) | ||
| 4 | 1 | reservoir | HEPES | 10 (mM) | |
| 5 | 1 | reservoir | 150 (mM) | ||
| 6 | 1 | reservoir | n-octyl beta-D-glucopyranoside | 2 (%) | |
| 7 | 1 | reservoir | PEG3350 | 3 (%) |
