1ATU
UNCLEAVED ALPHA-1-ANTITRYPSIN
Experimental procedure
| Source type | ROTATING ANODE |
| Source details | RIGAKU RUH3R |
| Temperature [K] | 293 |
| Detector technology | IMAGE PLATE |
| Collection date | 1995-07 |
| Detector | RIGAKU |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 115.780, 39.370, 90.430 |
| Unit cell angles | 90.00, 103.80, 90.00 |
Refinement procedure
| Resolution | 8.000 - 2.700 |
| R-factor | 0.195 |
| Rwork | 0.195 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 7api |
| RMSD bond length | 0.013 |
| RMSD bond angle | 26.120 * |
| Data reduction software | RIGAKU |
| Data scaling software | RIGAKU |
| Phasing software | X-PLOR |
| Refinement software | X-PLOR |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 8.000 | 3.000 |
| High resolution limit [Å] | 2.700 | 2.700 |
| Rmerge | 0.098 * | |
| Number of reflections | 9154 | |
| Completeness [%] | 83.5 * | 70.6 * |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion * | 6 | 20 * | drop solution was mixed with an equal volume of mother liquor * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | sodium phosphate | 50 (mM) | |
| 2 | 1 | drop | 100 (mM) | ||
| 3 | 1 | drop | EDTA | 1 (mM) | |
| 4 | 1 | drop | beta-mercaptoethanol | 1 (mM) | |
| 5 | 1 | drop | protein | 20 (mg/ml) | |
| 6 | 1 | reservoir | PEG4000 | 17-23 (%) | mother liquor |
| 7 | 1 | reservoir | isopropyl alcohol | 10 (%) | mother liquor |
