1ATI

CRYSTAL STRUCTURE OF GLYCYL-TRNA SYNTHETASE FROM THERMUS THERMOPHILUS

Experimental procedure

Source type	SYNCHROTRON
Source details	LURE BEAMLINE DW32
Synchrotron site	LURE
Beamline	DW32
Temperature [K]	277
Detector technology	IMAGE PLATE
Collection date	1992-11-10
Detector	MARRESEARCH
Spacegroup name	C 2 2 21
Unit cell lengths	126.900, 255.300, 105.000
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	20.000 - 2.750
R-factor	0.227
Rwork	0.227
R-free	0.29000
Structure solution method	MULTIPLE ISOMORPHOUS REPLACEMENT
RMSD bond length	0.014
RMSD bond angle	24.700 *
Data reduction software	MOSFLM
Data scaling software	CCP4 ((AGROVATA)
Phasing software	X-PLOR (3.1)
Refinement software	X-PLOR (3.1)

Data quality characteristics

	Overall
High resolution limit [Å]	2.900
Rmerge	0.010
Number of reflections	33033
Completeness [%]	90.9
Redundancy	4.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	7.5	15 *	Logan, D.T., (1994) J.Mol.Biol., 241, 732. *
1	Vapor diffusion, hanging drop *	7.5	15 *	Logan, D.T., (1994) J.Mol.Biol., 241, 732. *
2

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	GlyRS	6-8 (mg/ml)
2	1	reservoir	PEG6000	5-7 (%)
3	1	reservoir		0.8-1.4 (M)