1ASX

APICAL DOMAIN OF THE CHAPERONIN FROM THERMOPLASMA ACIDOPHILUM

Experimental procedure

Source type	ROTATING ANODE
Source details	RIGAKU RUH2R
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	1997-03
Detector	MARRESEARCH
Spacegroup name	P 31 2 1
Unit cell lengths	79.910, 79.910, 70.250
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	10.000 - 2.800
R-factor	0.222
Rwork	0.222
R-free	0.27200
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	GLOBULAR DOMAIN PORTION OF CRYSTAL FORM A (PDB ENTRY 1ASS)
RMSD bond length	0.011
RMSD bond angle	23.930 *
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	SOLOMON
Refinement software	X-PLOR (3.851)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	19.000
High resolution limit [Å]	2.800	2.800
Rmerge	0.064	0.145
Total number of observations	17354 *
Number of reflections	6335
<I/σ(I)>	20.6	6.9
Completeness [%]	94.8	71.8
Redundancy	2.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion *	3.8 *	30 *	pH 3.5

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	27 (mg/ml)
2	1	drop	ammonium sulfate	3.0 (M)
3	1	drop	sodium citrate	0.1 (M)