1ASV

Avian sarcoma virus integrase catalytic core domain

Experimental procedure

Temperature [K]	295
Detector technology	IMAGE PLATE
Collection date	1994-10-08
Detector	MARRESEARCH
Spacegroup name	P 43 21 2
Unit cell lengths	66.550, 66.550, 80.870
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	8.000 - 2.200
R-factor	0.142
R-free	0.23400
RMSD bond length	0.013
RMSD bond angle	0.045
Data reduction software	DENZO
Data scaling software	SCALEPACK
Refinement software	PROLSQ

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	25.000
High resolution limit [Å]	2.200
Rmerge	0.062
Number of reflections	9620
Completeness [%]	99.0	99.1 *
Redundancy	3.93

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	7.5 *		pH 5.6

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	reservoir	HEPES	100 (mM)
2	1	reservoir	isopropanol	10 (%)
3	1	drop	protein	6-7.5 (mg/ml)
4	1	reservoir	PEG4000	20 (%)