1ASU
AVIAN SARCOMA VIRUS INTEGRASE CATALYTIC CORE DOMAIN CRYSTALLIZED FROM 2% PEG 400, 2M AMMONIUM SULFATE, HEPES PH 7.5
Experimental procedure
| Temperature [K] | 295 |
| Detector technology | IMAGE PLATE |
| Collection date | 1994-11-19 |
| Detector | MAR scanner 300 mm plate |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 66.470, 66.470, 80.990 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 8.000 - 1.700 |
| R-factor | 0.152 |
| R-free | 0.18800 |
| RMSD bond length | 0.009 |
| RMSD bond angle | 0.035 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Refinement software | PROLSQ |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 25.000 |
| High resolution limit [Å] | 1.700 |
| Rmerge | 0.092 |
| Number of reflections | 18249 |
| Completeness [%] | 88.5 |
| Redundancy | 2.99 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | unknown * | 7.5 | pH 7.5 |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | reservoir | PEG4000 | 20 (%(w/v)) | |
| 2 | 1 | reservoir | HEPES | 100 (mM) | |
| 3 | 1 | reservoir | isopropanol | 10 (%) | |
| 4 | 1 | drop | protein | 6-7.5 (mg/ml) |
