1AS2

GDP+PI BOUND G42V GIA1

Experimental procedure

Source type	ROTATING ANODE
Source details	RIGAKU RUH3R
Temperature [K]	278
Detector technology	IMAGE PLATE
Collection date	1996-04
Detector	RIGAKU
Spacegroup name	P 43 21 2
Unit cell lengths	77.190, 77.190, 143.610
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	8.000 - 2.800
R-factor	0.189
Rwork	0.189
R-free	0.25000
Structure solution method	DIFFERENCE FOURIER
Starting model (for MR)	1git
RMSD bond length	0.012
RMSD bond angle	23.500 *
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	X-PLOR (3.1)
Refinement software	X-PLOR (3.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	15.000	2.930
High resolution limit [Å]	2.800	2.800
Rmerge	0.059	0.237
Number of reflections	10403
<I/σ(I)>	15.4
Completeness [%]	93.1	95.2
Redundancy	2.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5.6	20 *	THE PROTEIN WAS CRYSTALLIZED IN HANGING DROPS USING 3M (NH4)H2P04 AS THE PRECIPITANT (PH 5.6). 50 MM HEPES, PH 8.0, 10 MM MGSO4, 10 MM DTT AND 5 MM GDP WAS THE BUFFER, vapor diffusion - hanging drop

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	5-7.5 (mg/ml)
2	1	drop	ammonium phosphate	1.1-1.25 (M)
3	1	reservoir	ammonium phosphate	2.2-2.5 (M)