1ARX

CRYSTAL STRUCTURES OF CYANIDE-AND TRIIODIDE-BOUND FORMS OF ARTHROMYCES RAMOSUS PEROXIDASE AT DIFFERENT PH VALUES. PERTURBATIONS OF ACTIVE SITE RESIDUES AND THEIR IMPLICATION IN ENZYME CATALYSIS

Experimental procedure

Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE BL-6A
Synchrotron site	Photon Factory
Beamline	BL-6A
Detector technology	DIFFRACTOMETER
Detector	WEISSENBERG
Spacegroup name	P 42 21 2
Unit cell lengths	74.570, 74.570, 117.470
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	7.000 - 1.900
R-factor	0.169
Rwork	0.169
RMSD bond length	0.019
RMSD bond angle	0.052 *
Phasing software	X-PLOR
Refinement software	X-PLOR

Data quality characteristics

	Overall
High resolution limit [Å]	1.900 *
Rmerge	0.057 *
Total number of observations	77736 *
Number of reflections	20924 *
Completeness [%]	78.2 *

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	6.5	24 *	seeding *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	ARP	20-40 (mg/ml)
2	1	reservoir	ammonium sulfate	32 (%sat)
3	1	reservoir	Tris-HCl	20 (mM)