1ARS
X-RAY CRYSTALLOGRAPHIC STUDY OF PYRIDOXAL 5'-PHOSPHATE-TYPE ASPARTATE AMINOTRANSFERASES FROM ESCHERICHIA COLI IN OPEN AND CLOSED FORM
Experimental procedure
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 155.420, 87.130, 79.400 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 6.000 * - 1.800 |
| R-factor | 0.213 |
| Rwork | 0.213 |
| RMSD bond length | 0.016 |
| RMSD bond angle | 3.300 * |
| Phasing software | X-PLOR |
| Refinement software | X-PLOR |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 7 * | 20 * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | enzyme | 0.9 (mM) | |
| 10 | 1 | reservoir | sodium sulfate | 1 (M) | |
| 2 | 1 | drop | potassium phosphate | 10 (mM) | |
| 3 | 1 | drop | PLP | 0.01 (mM) | |
| 4 | 1 | drop | EDTA | 1 (mM) | |
| 5 | 1 | drop | 0.3 (mM) | ||
| 6 | 1 | reservoir | potassium phosphate | 10 (mM) | |
| 7 | 1 | reservoir | PLP | 0.01 (mM) | |
| 8 | 1 | reservoir | EDTA | 1 (mM) | |
| 9 | 1 | reservoir | 0.3 (mM) |
