1ARI
Aspartate aminotransferase, W140H mutant, maleate complex
Experimental procedure
| Temperature [K] | 277 |
| Detector technology | DIFFRACTOMETER |
| Collection date | 1994-01-19 |
| Detector | ENRAF-NONIUS FAST |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 86.400, 79.000, 90.800 |
| Unit cell angles | 90.00, 118.80, 90.00 |
Refinement procedure
| Resolution | 8.000 - 2.300 |
| R-factor | 0.21 |
| RMSD bond length | 0.012 |
| RMSD bond angle | 26.300 * |
| Data reduction software | MADNES |
| Refinement software | TNT (4C) |
Data quality characteristics
| Overall | |
| High resolution limit [Å] | 2.300 * |
| Rmerge | 0.171 |
| Redundancy | 1.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 7.7 * | pH 7.5 |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 10 (mg/ml) | |
| 2 | 1 | drop | sodium phosphate | 20 (mM) | |
| 3 | 1 | reservoir | PEG4000 | 30 (%) | |
| 4 | 1 | reservoir | sodium phosphate | 20 (mM) | |
| 5 | 1 | reservoir | 1.0 (M) | ||
| 6 | 1 | reservoir | maleate | 0.1 (M) | |
| 7 | 1 | reservoir | 0.02 (%) |
