1AQX
GLUTATHIONE S-TRANSFERASE IN COMPLEX WITH MEISENHEIMER COMPLEX
Experimental procedure
| Source type | ROTATING ANODE |
| Source details | RIGAKU RUH2R |
| Temperature [K] | 113 |
| Detector technology | IMAGE PLATE |
| Collection date | 1996-09-10 |
| Detector | MARRESEARCH |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 89.590, 72.570, 69.340 |
| Unit cell angles | 90.00, 90.11, 90.00 |
Refinement procedure
| Resolution | 7.000 - 2.000 |
| R-factor | 0.2015 |
| Rwork | 0.202 |
| R-free | 0.28300 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.009 |
| RMSD bond angle | 25.827 * |
| Data reduction software | MOSFLM |
| Data scaling software | CCP4 ((AGROVATA) |
| Phasing software | AMoRE |
| Refinement software | X-PLOR (3.5) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.269 | 2.080 |
| High resolution limit [Å] | 2.030 | 2.030 |
| Rmerge | 0.067 | 0.195 |
| Total number of observations | 100891 * | |
| Number of reflections | 52986 * | |
| Completeness [%] | 90.9 | |
| Redundancy | 1.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion * | 6.5 | 20 * | pH 6.5 |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | PEG8000 | 10 (%) | |
| 2 | 1 | drop | MES-NaOH | 33 (mM) | |
| 3 | 1 | drop | 67 (mM) | ||
| 4 | 1 | drop | protein | 13 (mg/ml) | |
| 5 | 1 | reservoir | PEG8000 | 30 (%) | |
| 6 | 1 | reservoir | MES-NaOH | 100 (mM) | |
| 7 | 1 | reservoir | 200 (mM) |
