1AQI
STRUCTURE OF ADENINE-N6-DNA-METHYLTRANSFERASE TAQI
Experimental procedure
| Source type | ROTATING ANODE |
| Source details | ENRAF-NONIUS FR571 |
| Detector technology | IMAGE PLATE |
| Collection date | 1994-04 |
| Detector | MARRESEARCH |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 132.080, 144.060, 53.440 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 10.000 - 2.600 |
| R-factor | 0.219 |
| Rwork | 0.219 |
| R-free | 0.28100 |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.230 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Refinement software | X-PLOR |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 10.000 |
| High resolution limit [Å] | 2.600 |
| Rmerge | 0.066 |
| Total number of observations | 110392 * |
| Number of reflections | 30844 |
| Completeness [%] | 96.0 |
| Redundancy | 3.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion * | 7.3 * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | M.TaqI | 0.00065 (mM) | |
| 2 | 1 | drop | Sinefungin | 1 (mM) | can be replaced by AdoHcy |
| 3 | 1 | drop | duplex oligonucleotides | 0.1 (mM) | |
| 4 | 1 | drop | 200 (mM) | ||
| 5 | 1 | drop | Tris-HCl | 20 (mM) | |
| 6 | 1 | reservoir | PEG6000 | 2-6 (%) | |
| 7 | 1 | reservoir | 100 (mM) | ||
| 8 | 1 | reservoir | Tris-HCl | 20 (mM) |
