1AQ4

STRUCTURE OF A MS2 COAT PROTEIN MUTANT IN COMPLEX WITH AN RNA OPERATOR

Experimental procedure

Source type	SYNCHROTRON
Source details	SRS BEAMLINE PX9.6
Synchrotron site	SRS
Beamline	PX9.6
Temperature [K]	290
Detector technology	IMAGE PLATE
Collection date	1995-05-28
Detector	MARRESEARCH
Spacegroup name	H 3 2
Unit cell lengths	288.000, 288.000, 653.000
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	10.000 - 3.000
R-factor	0.197
Rwork	0.197
R-free	0.25100 *
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	REFINED MODEL OF MS2 PROTEIN CAPSID
RMSD bond length	0.019
RMSD bond angle	28.000 *
Data reduction software	DENZO
Data scaling software	Agrovata
Refinement software	X-PLOR (3.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	25.000	3.080
High resolution limit [Å]	3.000	3.000
Rmerge	0.128	0.263
Number of reflections	190898	14247 *
<I/σ(I)>	4.2	2.6
Completeness [%]	92.3 *	95.1 *
Redundancy	3.3	3.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	7.4	37 *	pH 7.40

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	MS2	1.0 (%(w/v))
2	1	drop	sodium phosphate	0.2 (M)
3	1	drop	PEG6000	1.5 (%(w/v))
4	1	drop		0.02 (%(w/v))
5	1	reservoir	sodium phosphate	0.4 (M)