1APX

CRYSTAL STRUCTURE OF RECOMBINANT ASCORBATE PEROXIDASE

Experimental procedure

Detector technology	AREA DETECTOR
Collection date	1994-04-08
Detector	SIEMENS-NICOLET X100
Spacegroup name	C 1 2 1
Unit cell lengths	132.400, 53.000, 170.600
Unit cell angles	90.00, 107.10, 90.00

Refinement procedure

Resolution	8.000 - 2.200
R-factor	0.193
Rwork	0.193
RMSD bond length	0.007
RMSD bond angle	1.279 *
Data reduction software	XENGEN (V. 2.0)
Phasing software	X-PLOR (3.1)
Refinement software	X-PLOR (3.1)

Data quality characteristics

	Overall
Low resolution limit [Å]	9999.000 *
High resolution limit [Å]	1.900
Number of reflections	65092
Completeness [%]	72.0
Redundancy	2.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	8.5 *	25 *	or 7 degrees centigrade *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	10 (mg/ml)
2	1	reservoir	PEG4000	30 (%)
3	1	reservoir	sodium acetate	0.2 (M)
4	1	reservoir	Tris-HCl	0.1 (M)