1APN
THE CRYSTALLOGRAPHIC STRUCTURE OF METAL-FREE CONCANAVALIN A AT 2.5 ANGSTROMS RESOLUTION
Experimental procedure
| Detector technology | AREA DETECTOR |
| Collection date | 1993-01-20 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 61.360, 85.460, 91.460 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 10.000 - 2.500 |
| R-factor | 0.18 |
| Rwork | 0.180 |
| R-free | 0.24700 |
| RMSD bond length | 0.015 |
| RMSD bond angle | 26.510 * |
| Data reduction software | MADNESS |
| Phasing software | X-PLOR (3.1) |
| Refinement software | X-PLOR (3.1) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 15.000 |
| High resolution limit [Å] | 2.500 |
| Rmerge | 0.078 |
| Total number of observations | 88305 * |
| Number of reflections | 16834 |
| Completeness [%] | 98.0 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, sitting drop * | 5 * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 11 (mg/ml) | |
| 2 | 1 | reservoir | ammonium sulfate | 2 (M) | precipitant |
| 3 | 1 | reservoir | sodium acetate | 100 (mM) | precipitant |
| 4 | 1 | reservoir | PEG6000 | 5 (%) | precipitant |
| 5 | 1 | drop | precipitant |
