1APM

2.0 ANGSTROM REFINED CRYSTAL STRUCTURE OF THE CATALYTIC SUBUNIT OF CAMP-DEPENDENT PROTEIN KINASE COMPLEXED WITH A PEPTIDE INHIBITOR AND DETERGENT

Experimental procedure

Spacegroup name	P 21 21 21
Unit cell lengths	73.840, 75.760, 81.010
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	30.000 - 2.000
R-factor	0.186 *
Rwork	0.186
RMSD bond length	0.013
RMSD bond angle	2.300
Phasing software	X-PLOR
Refinement software	TNT

Data quality characteristics

	Overall
High resolution limit [Å]	1.950 *
Rmerge	0.056 *
Number of reflections	30428 *
Completeness [%]	90.0 *

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *		277 *	Zheng, J., (1992) Acta Crystallogr., B48, 241. *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	10 (mg/ml)
2	1	drop	ammonium acetate	150 (mM)
3	1	drop	2-mercaptoethanol	10 (mM)
4	1	drop	dithiothreitol	10 (mM)
5	1	reservoir	dithiothreitol	10 (mM)
6	1	reservoir	PEG400	8 (%(w/v))
7	1	reservoir	methanol	15 (%(v/v))