1AP5
TYR34->PHE MUTANT OF HUMAN MITOCHONDRIAL MANGANESE SUPEROXIDE DISMUTASE
Experimental procedure
Source type | SYNCHROTRON |
Source details | SSRL BEAMLINE BL7-1 |
Synchrotron site | SSRL |
Beamline | BL7-1 |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 1995-02 |
Detector | MARRESEARCH |
Spacegroup name | P 21 21 2 |
Unit cell lengths | 75.180, 79.770, 68.810 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 100.000 - 2.200 |
R-factor | 0.195 |
Rwork | 0.195 |
R-free | 0.32000 |
Structure solution method | ISOMORPHOUS REFINEMENT |
Starting model (for MR) | 1ABM |
RMSD bond length | 0.009 |
RMSD bond angle | 23.500 * |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | X-PLOR (3.8) |
Refinement software | X-PLOR (3.8) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 100.000 | 2.300 |
High resolution limit [Å] | 2.200 | 2.200 |
Number of reflections | 20748 | |
<I/σ(I)> | 8.5 | 2.5 |
Completeness [%] | 96.0 | 89 |
Redundancy | 3 | 3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Vapor diffusion, hanging drop * | 7.8 | Borgstahl, G.E.O., (1996) Biochemistry, 35, 4287. * |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | protein | 10 (mg/ml) | |
2 | 1 | reservoir | potassium phosphate | 25 (mM) | |
3 | 1 | reservoir | PEG4000 | 20 (%) |