1AOX

I DOMAIN FROM INTEGRIN ALPHA2-BETA1

Experimental procedure

Source type	ROTATING ANODE
Source details	RIGAKU
Temperature [K]	287
Detector technology	IMAGE PLATE
Collection date	1996-11
Detector	RIGAKU
Spacegroup name	P 1
Unit cell lengths	40.540, 43.290, 68.040
Unit cell angles	88.27, 76.59, 66.74

Refinement procedure

Resolution	15.000 - 2.100
R-factor	0.219
Rwork	0.219
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1ido
RMSD bond length	0.020
RMSD bond angle	26.000 *
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	X-PLOR (3.8)
Refinement software	X-PLOR (3.8)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.230
High resolution limit [Å]	2.100	2.100
Number of reflections	23946
<I/σ(I)>	21.8	9.7
Completeness [%]	99.5	99.5
Redundancy	3.2	3.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	7.5		Emsley, J., (1997) J. Biol. Chem., 272, 28512. *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	10 (mg/ml)
2	1	reservoir	PEG8000	16-20 (%)
3	1	reservoir	HEPES	50 (mM)
4	1	reservoir		10 (mM)
5	1	reservoir	glycerol	20 (%)